TY  - BOOK
AU  - Unkel, Katharina
PY  - 2025
DA  - 2025//
TI  - Three delivery methods of CRISPR/Cas9 to induce targeted mutations into centromeric histone H3 (CENH3) in carrots (Daucus carota L.): = Drei Transformationssysteme von CRISPR/Cas9 zur gezielten Veränderung vom zentromerspezifischen Histon H3 (CENH3) in Karotten (Daucus carota L.)
PB  - Universität Rostock
CY  - Rostock
AB  - The centromere-specific histone 3 (CENH3) in carrots (Daucus carota) was mutated using CRISPR/Cas9. Transformation with Agrobacterium tumefaciens and Agrobacterium rhizogenes resulted in transgenic lines with secondary mutations and lower levels of CENH3. Transient transformation of carrot protoplasts with CRISPR/Cas9 ribonucleoprotein complexes (RNPs) resulted in transgene-free and mutated protoplasts. The transgene-free method was identified as the preferred method due to the absence of secondary mutations.<eng>
UR  - https://purl.uni-rostock.de/rosdok/id00005439
UR  - https://doi.org/10.18453/rosdok_id00005439
DO  - 10.18453/rosdok_id00005439
LA  - English
N1  - vorgelegt von Katharina Unkel
ID  - 1969655291
ER  -