TY  - BOOK
AU  - Khani, Afsaneh
PY  - 2017
DA  - 2017//
TI  - Investigating the mechanism of a novel glycine-dependent riboswitch and a putative non-coding regulatory RNA in Streptococcus pyogenes
PB  - Universität Rostock
CY  - Rostock
AB  - We investigated gene expression regulation by a putative glycine(Gly) riboswitch located in the 5′-UTR of a SAF protein gene in the S.pyogenes. Gly-dependency was studied using a luciferase reporter gene system. Maximal reporter gene expression happened in the presence of low Gly concentrations. RT-qPCR showed that in the presence of Gly (≥1 mM), expression of the gene was downregulated. Growth in the presence of 0.1 mM Gly led to the production of a full-length transcript. We conclude that the Gly riboswitch in S. pyogenes represses gene expression in the presence of high Gly concentrations.<eng>
UR  - http://rosdok.uni-rostock.de/resolve/urn/urn:nbn:de:gbv:28-diss2018-0087-1
UR  - https://nbn-resolving.org/urn:nbn:de:gbv:28-diss2018-0087-1
UR  - https://d-nb.info/1293652717/34
UR  - https://doi.org/10.18453/rosdok_id00002093
DO  - 10.18453/rosdok_id00002093
LA  - English
N1  - vorgelegt von Afsaneh Khani aus dem Iran
ID  - 1025372573
ER  -