TY  - BOOK
AU  - Ni, Xiaoqi
PY  - 2016
DA  - 2016//
TI  - Electrochemical hybridization assay for DNA and asymmetric PCR products detection by labeling with osmium tetroxide
PB  - Universität Rostock
CY  - Rostock
AB  - This dissertation describes the hybridization assay on gold electrodes for electrochemical detection of DNA and polymerase chain reaction (PCR) products by means of the electrochemically active compound osmium tetroxide bipyridine. Single base mismatches can be discriminated for their different thermal behavior. We further optimized protective-strand design, surface-probe density, and hybridization temperature. Besides, we also investigated to obtain labeled single stranded DNA targets from asymmetric PCR after labeling forward primer with osmium tetroxide for electrochemical detection.<eng>
UR  - http://rosdok.uni-rostock.de/resolve/urn/urn:nbn:de:gbv:28-diss2018-0038-9
UR  - https://nbn-resolving.org/urn:nbn:de:gbv:28-diss2018-0038-9
UR  - https://d-nb.info/1293651869/34
UR  - https://doi.org/10.18453/rosdok_id00002044
DO  - 10.18453/rosdok_id00002044
LA  - English
N1  - vorgelegt von Xiaoqi Ni
ID  - 101834277X
ER  -