Aqueous two-phase extraction of proteins and enzymes using tetraalkylammonium-based ionic liquids
Dreyer, Susanne Elisabeth
1979-
aut
text
theses
Text
Hochschulschrift
gw
2008
monographic
XA-DE
2008
eng
text/html
application/pdf
Online-Ressource Ill., graph. Darst.
Within the scope of this thesis, the application of ionic liquids (IL) for the generation of aqueous two-phase systems (IL-based ATPS) and their use for the purification of catalytically active enzymes was evaluated. By studying the partitioning of different model proteins, a deeper understanding of the mechanisms influencing the partition process is provided. The IL AmmoengTM 110 in combination with an inorganic salt (K2HPO4/KH2PO4) can be used to establish an IL-based ATPS for the selective purification of two alcohol dehydrogenases. The presence of IL within the system was found to be advantageous due to its stability enhancing effect on both enzymes and its solubility enhancing effect on hydrophobic substrates.
vorgelegt von Susanne Elisabeth Dreyer
Rostock, Univ., Mathematisch-Naturwiss. Fak., Diss., 2009
660 540 570
35.69
http://rosdok.uni-rostock.de/resolve?urn=urn:nbn:de:gbv:28-diss2009-0038-8
http://rosdok.uni-rostock.de/resolve?urn=urn:nbn:de:gbv:28-diss2009-0038-8&pdf
http://nbn-resolving.de/urn:nbn:de:gbv:28-diss2009-0038-8
Aqueous two-phase extraction of proteins and enzymes using tetraalkylammonium-based ionic liquids
2008
Druck-Ausgabe
(DE-627)592166074
Dreyer, Susanne Elisabeth, 1979 -
urn:nbn:de:gbv:28-diss2009-0038-8
320543076
rakwb
DE-627
090226
592712125
20240710T004652.0
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