Increasing culturability of plant microbiome towards core microbiome manipulation and engineering

Increasing culturability of plant microbiome towards core microbiome manipulation and engineering Sarhan, Mohamed Sabry Mohamed 1993- VerfasserIn aut Eichler-Löbermann, Bettina 1968- AkademischeR BetreuerIn dgs Ruppel, Silke AkademischeR BetreuerIn dgs Franken, Philipp 1960-2025 AkademischeR BetreuerIn dgs Hegazi, Nabil AkademischeR BetreuerIn dgs Universität Rostock Grad-verleihende Institution dgg Universität Rostock Agrar- und Umweltwissenschaftliche Fakultät Grad-verleihende Institution dgg text theses Text Hochschulschrift gw 2020 monographic XA-DE Rostock Universität publisher 2020 Rostock Universitätsbibliothek distributor eng 1 Online-Ressource This study aimed to optimize the use of plant-based culture media for exploration of the plant-associated microbiota and extend the culturability borders to isolate novel bacterial taxa and meticulously characterize the culture-dependent and culture-independent microbiomes in view of the developed plant-based culture media. Throughout the study, we could prove that plant-based culture media are able to support the recovery of previously uncultured plant-associated bacteria, and also support the enrichment of bacterial lineages that belong to the candidate phyla radiation (CPR).<eng> vorgelegt von Mohamed Sabry Mohamed Sarhan Enthält Zeitschriftenartikel Articles included: Chapter 2: Hegazi, N.A., Sarhan, M.S., Fayez, M., Patz, S., Murphy, B.R. and Ruppel, S., 2017. Plant-fed versus chemicals-fed rhizobacteria of Lucerne: Plant-only teabags culture media not only increase culturability of rhizobacteria but also recover a previously uncultured Lysobacter sp., Novosphingobium sp. and Pedobacter sp. PloS one, 12(7), p.e0180424.https://doi.org/10.1371/journal.pone.0180424 Chapter 3: Sarhan, M.S., Mourad, E.F., Nemr, R.A., Abdelfadeel, M.R., Daanaa, H.S.A., Youssef, H.H., Goda, H.A., Hamza, M.A., Fayez, M., Eichler-Löbermann, B. and Ruppel, S., 2019. An inoculum-dependent culturing strategy (IDC) for the cultivation of environmental microbiomes and the isolation of novel endophytic Actinobacteria. The Journal of antibiotics, pp.1-6. https://doi.org/10.1038/s41429-019-0226-4 Chapter 4: Sarhan, M.S., Patz, S., Hamza, M.A., Youssef, H.H., Mourad, E.F., Fayez, M., Murphy, B., Ruppel, S. and Hegazi, N.A., 2018. G3 PhyloChip Analysis Confirms the Promise of Plant-Based Culture Media for Unlocking the Composition and Diversity of the Maize Root Microbiome and for Recovering Unculturable Candidate Divisions/Phyla. Microbes and environments, 33 (3) pp 317-325. https://doi.org/10.1264/jsme2.ME18023 Chapter 5: Sarhan, M.S., Hamza, M.A., Youssef, H.H., Patz, S., Becker, M., ElSawey, H., Nemr, R., Daanaa, H.S.A., Mourad, E.F., Morsi, A.T. and Abdelfadeel, M.R., 2019. Culturomics of the plant prokaryotic microbiome and the dawn of plant-based culture media–A review. Journal of advanced research,19 (September) pp 15-27. https://doi.org/10.1016/j.jare.2019.04.002 GutachterInnen: Bettina Eichler-Löbermann (Universität Rostock) ; Silke Ruppel (Leibniz-Institut für Gemüse- und Zierpflanzenbau (IGZ) e.V., Großbeeren) ; Philipp Franken (Universität Jena) ; Nabil Hegazi (Cairo University, ÄGYPTEN) Dissertation Universität Rostock 2020 Kumulative Dissertation 570 580 630 570 ZC 10000 48.50 http://purl.uni-rostock.de/rosdok/id00003155 https://doi.org/10.18453/rosdok_id00003155 https://nbn-resolving.org/urn:nbn:de:gbv:28-rosdok_id00003155-0 https://d-nb.info/1293534552/34 Increasing culturability of plant microbiome towards core microbiome manipulation and engineering Rostock, 2020 Druck-Ausgabe (DE-627)1773180193 Sarhan, Mohamed Sabry Mohamed, 1993 - urn:nbn:de:gbv:28-rosdok_id00003155-0 10.18453/rosdok_id00003155 1268266448 rda DE-627 210916 1770835318 20251226T194839.0 Converted from MARCXML to MODS version 3.8 using MARC21slim2MODS3-8_XSLT1-0.xsl (Revision 1.174 20250328) ger