Electrochemical hybridization assay for DNA and asymmetric PCR products detection by labeling with osmium tetroxide
Ni, Xiaoqi
1984-
VerfasserIn
aut
Flechsig, Gerd-Uwe
1972-
GutachterIn
oth
Langer, Peter
1969-
GutachterIn
oth
Universität Rostock
Grad verleihende Institution
dgg
Universität Rostock
Mathematisch-Naturwissenschaftliche Fakultät
Grad verleihende Institution
dgg
text
theses
Text
Hochschulschrift
gw
2016
monographic
XA-DE
Rostock
Universität
publisher
[2016]
eng
1 Online-Ressource
This dissertation describes the hybridization assay on gold electrodes for electrochemical detection of DNA and polymerase chain reaction (PCR) products by means of the electrochemically active compound osmium tetroxide bipyridine. Single base mismatches can be discriminated for their different thermal behavior. We further optimized protective-strand design, surface-probe density, and hybridization temperature. Besides, we also investigated to obtain labeled single stranded DNA targets from asymmetric PCR after labeling forward primer with osmium tetroxide for electrochemical detection.<eng>
vorgelegt von Xiaoqi Ni
Dissertation Universität Rostock 2017
547.7
572.8636
540
543
540 570
35.39
http://rosdok.uni-rostock.de/resolve/urn/urn:nbn:de:gbv:28-diss2018-0038-9
https://doi.org/10.18453/rosdok_id00002044
https://nbn-resolving.org/urn:nbn:de:gbv:28-diss2018-0038-9
https://d-nb.info/1293651869/34
Electrochemical hybridization assay for DNA and asymmetric PCR products detection by labeling with osmium tetroxide
Rostock : Universität, 2016
Druck-Ausgabe
(DE-627)87890168X
Ni, Xiaoqi, 1984 -
urn:nbn:de:gbv:28-diss2018-0038-9
10.18453/rosdok_id00002044
1030989834
rda
DE-627
180411
101834277X
20251224T211552.0
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