%0 Book
%T Three delivery methods of CRISPR/Cas9 to induce targeted mutations into centromeric histone H3 (CENH3) in carrots (Daucus carota L.): = Drei Transformationssysteme von CRISPR/Cas9 zur gezielten Veränderung vom zentromerspezifischen Histon H3 (CENH3) in Karotten (Daucus carota L.)
%A Unkel, Katharina
%D 2025
%C Rostock
%C Universität Rostock
%G English
%F 1969655291
%O vorgelegt von Katharina Unkel
%O GutachterInnen: Martin Hagemann (Universität Rostock) ; Inge Broer (Universität Rostock) ; Frank Hartung (Julius Kühn-Institut)
%O Dissertation Universität Rostock 2025
%X The centromere-specific histone 3 (CENH3) in carrots (Daucus carota) was mutated using CRISPR/Cas9. Transformation with Agrobacterium tumefaciens and Agrobacterium rhizogenes resulted in transgenic lines with secondary mutations and lower levels of CENH3. Transient transformation of carrot protoplasts with CRISPR/Cas9 ribonucleoprotein complexes (RNPs) resulted in transgene-free and mutated protoplasts. The transgene-free method was identified as the preferred method due to the absence of secondary mutations.<eng>
%L 570
%9 theses
%9 Text
%9 Hochschulschrift
%R 10.18453/rosdok_id00005439
%U https://purl.uni-rostock.de/rosdok/id00005439
%U https://doi.org/10.18453/rosdok_id00005439