%0 Book
%T Establishment of a human 3D kidney tissue model using decellularized rat kidney slices
%A Salti, Haitham
%D 2024
%C Rostock
%C Universität Rostock
%G English
%F 194143438X
%O vorgelegt von Haitham Salti
%O GutachterInnen: Steffen Mitzner (Universitätsmedizin Rostock, Zentrum für Innere Medizin, Sektion Nephrologie) ; Kirsten Peters (Universitätsmedizin Rostock, Zentrum für Medizinische Forschung, Institut für Zellbiologie) ; Kerstin Amann (Uniklinik Erlangen, Nephropathologische Abteilung)
%O Dissertation Universität Rostock 2024
%X This study explores physical pretreatments to improve kidney decellularization, addressing the need for a bioartificial kidney as an alternative treatment for end-stage renal disease. Freezing-thawing cycles (FTC) and high hydrostatic pressure (HHP) were compared to chemical decellularization. FTC was the most effective in removing DNA and preserving the extracellular matrix (ECM), and showed better scaffold recellularization with kidney cells. Analysis with artificial intelligence revealed insights into the instructive memory of the ECM, guiding cells to avoid attachment to nonspecific sites.<eng>
%L 570
%9 theses
%9 Text
%9 Hochschulschrift
%R 10.18453/rosdok_id00005005
%U https://purl.uni-rostock.de/rosdok/id00005005
%U https://nbn-resolving.org/urn:nbn:de:gbv:28-rosdok_id00005005-3
%U https://d-nb.info/1382476930/34
%U https://doi.org/10.18453/rosdok_id00005005