%0 Book
%T Investigating the mechanism of a novel glycine-dependent riboswitch and a putative non-coding regulatory RNA in Streptococcus pyogenes
%A Khani, Afsaneh
%D 2017
%C Rostock
%C Universität Rostock
%G English
%F 1025372573
%O vorgelegt von Afsaneh Khani aus dem Iran
%O Dissertation Universität Rostock 2018
%X We investigated gene expression regulation by a putative glycine(Gly) riboswitch located in the 5′-UTR of a SAF protein gene in the S.pyogenes. Gly-dependency was studied using a luciferase reporter gene system. Maximal reporter gene expression happened in the presence of low Gly concentrations. RT-qPCR showed that in the presence of Gly (≥1 mM), expression of the gene was downregulated. Growth in the presence of 0.1 mM Gly led to the production of a full-length transcript. We conclude that the Gly riboswitch in S. pyogenes represses gene expression in the presence of high Gly concentrations.<eng>
%L 570
%9 theses
%9 Text
%9 Hochschulschrift
%R 10.18453/rosdok_id00002093
%U http://rosdok.uni-rostock.de/resolve/urn/urn:nbn:de:gbv:28-diss2018-0087-1
%U https://nbn-resolving.org/urn:nbn:de:gbv:28-diss2018-0087-1
%U https://d-nb.info/1293652717/34
%U https://doi.org/10.18453/rosdok_id00002093