Genetic characterization, heterologous expression and application of S-layer proteins from the bacterial isolates Lysinibacillus sphaericus JG-B53 and Lysinibacillus sphaericus JG-A12 Lederer, Franziska , 1983- Text theses Text Hochschulschrift 2012 2012 eng electronic resourceremoteComputermedienOnline-Ressourcetext/htmlapplication/pdfOnline-Ressource Ill., graph. Darst. The bacterial isolates Lysinibacillus sphaericus JG-A12 and JG-B53 were isolated from a uranium mining waste pile that is contaminated with multiple heavy metal ions. Genome analyses of Bacillus sp. JG-B53 were used to identify bacterial strategies to handle heavy metal polluted environments. The heterologous expression of the silent S-layer like protein gene sllB of L. sphaericus JG-A12 in E. coli induced distinct morphological modifications. Investigations identified SllB, to inhibit bacterial cell division. The filamentous structures were used for the design of polyelectrolyte capsules. vorgelegt von Franziska Linda Lederer Die Online-Ausg. weicht von der gedr. Ausg. ab. Teile der Diss., die nur die gedr. Ausg. enthält, werden in identischer Form in Publikationen veröffentlicht Rostock, Univ., Mathematisch-Naturwiss. Fak., Diss., 2013 WG 4100 42.30 http://rosdok.uni-rostock.de/resolve?urn=urn:nbn:de:gbv:28-diss2013-0032-9 http://rosdok.uni-rostock.de/resolve?urn=urn:nbn:de:gbv:28-diss2013-0032-9&pdf http://nbn-resolving.de/urn:nbn:de:gbv:28-diss2013-0032-9 Genetic characterization, heterologous expression and application of S-layer proteins from the bacterial isolates Lysinibacillus sphaericus JG-B53 and Lysinibacillus sphaericus JG-A12--(DE-627)738368571 urn:nbn:de:gbv:28-diss2013-0032-9 oclc: 935000334 ppn: (DE-627)739961047