Streptamer technology allows to isolate leukemia antigen-specific CD8+ T cells Wang, Xinchao , 1969- Text theses Text Hochschulschrift 2009 2009 eng electronic resourceremoteComputermedienOnline-Ressourcetext/htmlapplication/pdfOnline-Ressource graph. Darst. In this work we investigated whether streptamer technology could purify WT1-specific CD8+ T cells, what is important for the development of adoptive immunotherapy. Sample from HLA/A2+ HDs were identified and selected by streptamer. The function of selected CD8+ T cells was identified by the staining of phenotypic markers. The results showed that streptamer permits the detection and selection of WT1-specific CD8+ T cells in the PBMCs from HDs. The naïve function of selected CD8+ T cells was preserved and most selected CD8+ T cells demonstrated an effector T cell immunophenotype. presented by Xinchao Wang Rostock, Univ., Med. Fak., Diss., 2009 610 44.45 42.13 http://rosdok.uni-rostock.de/resolve?urn=urn:nbn:de:gbv:28-diss2009-0196-0 http://rosdok.uni-rostock.de/resolve?urn=urn:nbn:de:gbv:28-diss2009-0196-0&pdf http://nbn-resolving.de/urn:nbn:de:gbv:28-diss2009-0196-0 http://rosdok.uni-rostock.de/file/rosdok_derivate_000000004099/Dissertation_Wang_2009.pdf http://d-nb.info/99976232X/34 http://nbn-resolving.de/urn:nbn:de:gbv:28-diss2009-0196-0 Streptamer technology allows to isolate leukemia antigen-specific CD8+ T cells--(DE-627)612949389 urn:nbn:de:gbv:28-diss2009-0196-0 oclc: 643166491 ppn: (DE-627)613868986