Aqueous two-phase extraction of proteins and enzymes using tetraalkylammonium-based ionic liquids Dreyer, Susanne Elisabeth , 1979- Text theses Text Hochschulschrift 2008 2008 eng electronic resourceremoteComputermedienOnline-Ressourcetext/htmlapplication/pdfOnline-Ressource Ill., graph. Darst. Within the scope of this thesis, the application of ionic liquids (IL) for the generation of aqueous two-phase systems (IL-based ATPS) and their use for the purification of catalytically active enzymes was evaluated. By studying the partitioning of different model proteins, a deeper understanding of the mechanisms influencing the partition process is provided. The IL AmmoengTM 110 in combination with an inorganic salt (K2HPO4/KH2PO4) can be used to establish an IL-based ATPS for the selective purification of two alcohol dehydrogenases. The presence of IL within the system was found to be advantageous due to its stability enhancing effect on both enzymes and its solubility enhancing effect on hydrophobic substrates. vorgelegt von Susanne Elisabeth Dreyer Rostock, Univ., Mathematisch-Naturwiss. Fak., Diss., 2009 660 540 570 35.69 http://rosdok.uni-rostock.de/resolve?urn=urn:nbn:de:gbv:28-diss2009-0038-8 http://rosdok.uni-rostock.de/resolve?urn=urn:nbn:de:gbv:28-diss2009-0038-8&pdf http://nbn-resolving.de/urn:nbn:de:gbv:28-diss2009-0038-8 Aqueous two-phase extraction of proteins and enzymes using tetraalkylammonium-based ionic liquids--(DE-627)592166074 urn:nbn:de:gbv:28-diss2009-0038-8 oclc: 320543076 ppn: (DE-627)592712125