Electrochemical hybridization assay for DNA and asymmetric PCR products detection by labeling with osmium tetroxide Ni, Xiaoqi , 1984- (VerfasserIn) Flechsig, Gerd-Uwe , 1972- (GutachterIn) Langer, Peter , 1969- (GutachterIn) Universität Rostock (Grad verleihende Institution) Universität Rostock Mathematisch-Naturwissenschaftliche Fakultät (Grad verleihende Institution) Text theses Text Hochschulschrift 2016 [2016] eng electronic resourceremoteComputermedienOnline-Ressource1 Online-Ressource This dissertation describes the hybridization assay on gold electrodes for electrochemical detection of DNA and polymerase chain reaction (PCR) products by means of the electrochemically active compound osmium tetroxide bipyridine. Single base mismatches can be discriminated for their different thermal behavior. We further optimized protective-strand design, surface-probe density, and hybridization temperature. Besides, we also investigated to obtain labeled single stranded DNA targets from asymmetric PCR after labeling forward primer with osmium tetroxide for electrochemical detection.<eng> vorgelegt von Xiaoqi Ni Dissertation Universität Rostock 2017 547.7 572.8636 540 543 540 570 35.39 http://rosdok.uni-rostock.de/resolve/urn/urn:nbn:de:gbv:28-diss2018-0038-9 https://doi.org/10.18453/rosdok_id00002044 https://nbn-resolving.org/urn:nbn:de:gbv:28-diss2018-0038-9 https://d-nb.info/1293651869/34 Electrochemical hybridization assay for DNA and asymmetric PCR products detection by labeling with osmium tetroxide--(DE-627)87890168X urn:nbn:de:gbv:28-diss2018-0038-9 doi: 10.18453/rosdok_id00002044 oclc: 1030989834 ppn: (DE-627)101834277X