Establishment of a novel in vitro implant infection model: effects of electrical stimulation on staphylococci using alternating current Dauben, Thomas Josef , 1989- (VerfasserIn) Kreikemeyer, Bernd , 1966- (GutachterIn) Bahl, Hubert , 1955- (GutachterIn) Bader, Rainer , 1965- (GutachterIn) Universität Rostock (Grad-verleihende Institution) Universität Rostock Mathematisch-Naturwissenschaftliche Fakultät (Grad-verleihende Institution) Text theses Text Hochschulschrift 2017 2017 eng electronic resourceremoteComputermedienOnline-Ressource1 Online-Ressource An in vitro system for electrical stimulation of cells and bacteria was developed. Stimulation of cells and bacteria can be conducted under similar conditions. Prior to experiments, numerical simulations were performed. Methods for evaluation of effects were established. Stimulation showed significant effects on bacterial growth and biofilm mass production. Following stimulation, changes in extracellular DNA amounts were detected. In co-culture, stimulation led to increased CFU/ml S. epidermidis in supernatants, on and into MG63 cells on electrode surfaces and chamber bottom.<eng> vorgelegt von: Thomas Josef Dauben aus Rostock Dissertation Universität Rostock 2018 570 616.9 610 XD 4600 42.15 44.43 http://rosdok.uni-rostock.de/resolve/urn/urn:nbn:de:gbv:28-diss2018-0031-8 https://doi.org/10.18453/rosdok_id00002037 https://nbn-resolving.org/urn:nbn:de:gbv:28-diss2018-0031-8 https://d-nb.info/1293651737/34 Establishment of a novel in vitro implant infection model--(DE-627)1014910544 urn:nbn:de:gbv:28-diss2018-0031-8 doi: 10.18453/rosdok_id00002037 oclc: 1029251262 ppn: (DE-627)1016258844