@Book{1969655291,
author="Unkel, Katharina",
title="Three delivery methods of CRISPR/Cas9 to induce targeted mutations into centromeric histone H3 (CENH3) in carrots (Daucus carota L.): = Drei Transformationssysteme von CRISPR/Cas9 zur gezielten Ver{\"a}nderung vom zentromerspezifischen Histon H3 (CENH3) in Karotten (Daucus carota L.)",
year="2025",
address="Rostock",
abstract="The centromere-specific histone 3 (CENH3) in carrots (Daucus carota) was mutated using CRISPR/Cas9. Transformation with Agrobacterium tumefaciens and Agrobacterium rhizogenes resulted in transgenic lines with secondary mutations and lower levels of CENH3. Transient transformation of carrot protoplasts with CRISPR/Cas9 ribonucleoprotein complexes (RNPs) resulted in transgene-free and mutated protoplasts. The transgene-free method was identified as the preferred method due to the absence of secondary mutations.<eng>",
school="Universit{\"a}t Rostock",
note="vorgelegt von Katharina Unkel",
note="GutachterInnen: Martin Hagemann (Universit{\"a}t Rostock) ; Inge Broer (Universit{\"a}t Rostock) ; Frank Hartung (Julius K{\"u}hn-Institut)",
note="Dissertation Universit{\"a}t Rostock 2025",
doi="10.18453/rosdok_id00005439",
url="https://purl.uni-rostock.de/rosdok/id00005439",
url="https://doi.org/10.18453/rosdok_id00005439",
language="English"
}